Swati Tiwari
Pondicherry University, Puducherry
Title: Emulsion based delivery system for ‘Green’ carotenoids extracted from carrot bio-waste
Biography
Biography: Swati Tiwari
Abstract
Carotenoids extracted from carrot pomace (CREP) in oil using green bio-refinery approach were incorporated into the emulsion based delivery system. The oil in water emulsion was prepared by varying the levels of carotenoid rich extract (30-40%), whey protein concentrates (WPC-80) (5-10%) and lactose (5-10%), while keeping the level of gum arabic constant. The different combinations were analyzed for particle size, zeta potential, viscosity and colour value (L*, a* and b*). The optimized emulsion was analysed for total carotenoid content, antioxidant activity as ABTS, DPPH, FRAP and colour value.
Findings: The optimized emulsion yielded a particle size of 120.03±8.20 nm, zeta potential of -16.57±0.49mV, viscosity of 0.124±0.0115 Pa.s, L*
of 75.11±0.04, a* of 9.66±0.32 and b* value of 50.29±0.62. The encapsulation efficiency of the optimized emulsion was approx. 92.745 %. The emulsion had total carotenoid content of 34.6±0.137 (μg/g), antioxidant activity in terms of ABTS 1133.28±25.67 μg Trolox eq./ml, DPPH 292.07±23.22 μg Trolox eq./ml and FRAP 622.96±18.91 μM Trolox eq./ml and color value in terms of L* 74.21±0.32, a* 10.12±0.342 and b* 51.94±0.450. The liquid emulsion was observed under Transmission Electron Microscopy, while the freeze dried form of emulsion was observed under Scanning Electron Microscopy.